技术专题
晒科研、赢奖金| 抗体定制精彩案例回顾
文章来源：genecreate
作者：genecreate
发布时间：2020-06-01 16:45
经常做免疫实验的科研君都知道一支好抗体与实验的成败息息相关！但想买到适合自己课题的好抗体又很难，大多数科研君首先想到的是购买市场现有的目录产品抗体。然而目录产品抗体常常因为针对性差或其他各式各样的问题在课题研究中不奏效或者实验结果差强人意。这个时候才想到去专业公司定制化制备一支抗体，不仅浪费了大量金钱也耽搁了宝贵时间......遇到事情不要慌，且听小编一言：金开瑞抗体定制服务,让您的抗体研究开启freestyle！
经典案例回顾
案例一
comparative proteomics combined with analyses of transgenic plants reveal zmrem1.3 mediates maize resistance to southern corn rust. plant biotechnology journal.
合作技术：抗体制备
抗体：zmrem1.3兔多抗
western blot analysis of zmrem1.3 in transgenic maize lines 227, 229, 231 and 233 as well as the corresponding non-transgenic sib lines, with b-actin as the loading control.
案例二
in-depth proteome of the hypopharyngeal glands of honeybee workers reveals highly activated protein and energy metabolism in priming the secretion of royal jelly.
合作技术：抗体制备
抗体：多种兔多抗和鼠单抗
polyclonal rabbit antibodies against 60s ribosomal proteins rpl28, rpl26, and 40s ribosomal protein s4 (rps4), and the monoclonal mouse antibody against major royal jelly protein 1 (mrjp1) were developed (genecreate biological engineering, wuhan, china).
案例三
divergent molecular evolution in glutathione s-transferase conferring malathionresistance in the oriental fruit fly, bactrocera dorsalis (hendel).
合作技术：抗体制备
抗体：制备mr、ms特异性抗体
案例四
crd1, an xpo1 domain protein, regulates mirna accumulation and crown root development in rice, plant journal.
合作技术：抗体制备
抗体：抗crd 1多克隆抗体
(c) immunostaining of crd1-gfp (red fluorescence) in cross-sections of root tips of hj2 (upper panel) and crd1-gfp lines (lower panel). (d) validation of crd1 antibody (anti-crd1) using immunoblot analysis. (e) subcellular localization of crd1 in hj2 analyzed by immunoblot analysis.
案例五
multiplex immunoassay of chicken cytokines via highly-sensitive chemiluminescent imaging array，analytica chimica acta.
合作技术：抗体制备
抗体：chil-4和chifn-4的单抗、纯化的重组chil-4和chifn-1抗原
for the first incubation, the surface capture of the cytokines by their respective primary antibodies anti-chil-4 and anti-chifn-γ, the subsequent cl intensity value increased with increasing incubation time up to a maximum at 30 min (fig.2a). for the second step, formation of the sandwich via the attachment of the ab2-aunp-hrp probe to the exposed ab1-cytokine-immunocomplex, the maximum cl value was similarly reached with only a 25 min incubation (fig.2b).
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